Isolation and characterization of cDNA clones for human liver fibronectin
- 1 July 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 186 (1) , 31-34
- https://doi.org/10.1016/0014-5793(85)81333-8
Abstract
Cellular and plasma fibronectin dimers are constituted by similar but not identical polypeptides. Their differences are the consequence of internal primary sequence variability due to alternative splicing in at least 2 regions (ED and IIICS) of the pre mRNAs [1-8]. A detailed analysis of human liver fibronectin mRNA in these regions was carried out by isolating cDNA clones and determining their nucleotide sequence. A novel type of IIICS segment (coding for 64 amino acids) was present in the two cDNA clones studied and, as expected from previous S1 mapping studies [6], the ED segment was absent in both.Keywords
This publication has 16 references indexed in Scilit:
- A single rat fibronectin gene generates three different mRNAs by alternative splicing of a complex exon.Proceedings of the National Academy of Sciences, 1984
- Human fibronectin: cell specific alternative mRNA splicing generates polypeptide chains dfffering in the number of internal repeatsNucleic Acids Research, 1984
- The structural basis of the multiple forms of human complement component C4Cell, 1984
- Three different fibronectin mRNAs arise by alternative splicing within the coding regionCell, 1983
- Isolation and characterization of cDNA clones for human and bovine fibronectins.Proceedings of the National Academy of Sciences, 1983
- Partial primary structure of bovine plasma fibronectin: Three types of internal homologyProceedings of the National Academy of Sciences, 1983
- NH2-terminal sequences of DNA-, heparin-, and gelatin-binding tryptic fragments from human plasma fibronectinArchives of Biochemistry and Biophysics, 1982
- Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencingJournal of Molecular Biology, 1980
- Filter replicas and permanent collections of recombinant DNA plasmidsNucleic Acids Research, 1979
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977