Acetylcholine release from guinea pig caudate slices evoked by phorbol ester and calcium
Open Access
- 20 January 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 195 (1-2) , 129-134
- https://doi.org/10.1016/0014-5793(86)80146-6
Abstract
In guinea pig caudate slices preloaded with [3H]choline, superfusion with medium containing Ca2+ and the phorbol ester TPA (12‐O‐tetradecanoyl phorbol 13‐acetate) produced an increase in the Ca2+‐dependent, depolarization‐evoked Ca2+ influx. The effect of TPA was antagonized by polymyxin B and H‐7, both inhibitors of protein kinase C. The combination of TPA and calcium ionophore (A23187) provoked the release of ACh to a level equal to the maximal response induced by depolarizing stimuli. TPA alone had no significant effect on the release of ACh. These results suggest that protein kinase C probably plays a role in transmembrane signal transduction involved in the release of neurotransmitter from nerve terminals in the brain.Keywords
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