The Localization of Oat (Avena sativaL.) Seed Globulins in Protein Bodies

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Abstract
The major storage proteins of the oat grain are the 12S and 7S globulins. Using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immuno-difTusion assays we have demonstrated that the 7S globulin is localized predominantly in the embryo and the 12S globulin chiefly in the endosperm. Protein bodies have been isolated using aqueous and non-aqueous media and sucrose density gradient ultracentrifugation. Assaying marker enzymes gave results consistent with the presence of vacuolar protein bodies in the preparations. SDS-PAGE of sequential salt and alcohol extractions demonstrated the presence of globulins and prolamins respectively and their distribution within the gradient suggested that they may be localized in different protein bodies.