Metabolism of Bovine Semen. X. Oxidation of Carboxy-C14-Labeled Fatty Acids by Bovine Spermatozoa

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Abstract
C14 labeled fatty acids were used to show that washed bovine spermatozoa oxidize acetate, octanoate and butyrate readily; the other acids tested (myristate, stearate, palmitate and formate) were converted to C14O2 at slower but appreciable rates. The production of C14O2 from octanoate-1-C14 was shown to be oxygen-dependent and blocked by azide, cyanide and malonate but stimulated by 2, 4-dinitrophenol and coenzyme A. Glucose and glycerol did not appear to be competitive with octanoate as substrates for spermatozoa, but the presence of unlabeled aGetate, propionate or butyrate caused some reduction in utilization of octanoate-1-C14.