Partial purification procedure for human urinary erythropoietin by preparative isotachophoresis
- 1 October 1978
- journal article
- research article
- Published by Springer Nature in Zeitschrift für Die Gesamte Experimentelle Medizin
- Vol. 173 (3) , 293-296
- https://doi.org/10.1007/bf01851501
Abstract
Summary Human urinary erythropoietic-active crude protein was isotachophoretic fractionated in a LKB Uniphor apparatus equipped with a plastic column using polyacrylamide as a stabilizing medium, phosphat ions as leading and glycinat ions as terminating electrolytes and Ampholine carrier ampholytes (pH: 5–7) as spacers. The exhypoxic polycythemic mouse method to assay erythropoietic activity showed that the majority of the hormon was sharply resolved. Under these conditions preparative isotachophoresis demonstrated a higher purification factor compared to other biochemical preparation-methods.Keywords
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