Homodimeric galectin-7 (p53-induced gene 1) is a negative growth regulator for human neuroblastoma cells
Open Access
- 18 September 2003
- journal article
- research article
- Published by Springer Nature in Oncogene
- Vol. 22 (40) , 6277-6288
- https://doi.org/10.1038/sj.onc.1206631
Abstract
The extracellular functions of galectin-7 (p53-induced gene 1) are largely unknown. On the surface of neuroblastoma cells (SK-N-MC), the increased GM1 density, a result of upregulated ganglioside sialidase activity, is a key factor for the switch from proliferation to differentiation. We show by solid-phase and cell assays that the sugar chain of this ganglioside is a ligand for galectin-7. In serum-supplemented proliferation assays, galectin-7 reduced neuroblastoma cell growth without the appearance of features characteristic for classical apoptosis. The presence of galectin-3 blocked this effect, which mechanistically resembles that of galectin-1. By virtue of carbohydrate binding, galectin-7 thus exerts neuroblastoma growth control similar to galectin-1 despite their structural differences. In addition to p53-linked proapoptotic activity intracellularly, galectin-7, acting as a lectin on the cell surface, appears to be capable of reducing cancer cell proliferation in susceptible systems.Keywords
This publication has 88 references indexed in Scilit:
- Binding and cross-linking properties of galectinsBiochimica et Biophysica Acta (BBA) - General Subjects, 2002
- Thermodynamic binding studies of cell surface carbohydrate epitopes to galectins-1, -3, and -7: Evidence for differential binding specificitiesCanadian Journal of Chemistry, 2002
- Galectin-1 Modulates Human Glioblastoma Cell Migration into the Brain Through Modifications to the Actin Cytoskeleton and Levels of Expression of Small GTPasesJournal of Neuropathology and Experimental Neurology, 2002
- Wedgelike Glycodendrimers as Inhibitors of Binding of Mammalian Galectins to Glycoproteins, Lactose Maxiclusters, and Cell Surface GlycoconjugatesChemBioChem, 2001
- A mutant cholera toxin B subunit that binds GM1- ganglioside but lacks immunomodulatory or toxic activityProceedings of the National Academy of Sciences, 2001
- Galectins Are Differentially Expressed in Supratentorial Pilocytic Astrocytomas, Astrocytomas, Anaplastic Astrocytomas and Glioblastomas, and Significantly Modulate Tumor Astrocyte MigrationBrain Pathology, 2001
- Lectin-Mediated Drug Targeting: Selection of Valency, Sugar Type (Gal/Lac), and Spacer Length for Cluster Glycosides as Parameters to Distinguish Ligand Binding to C-Type Asialoglycoprotein Receptors and GalectinsPharmaceutical Research, 2000
- Galectin-7 overexpression is associated with the apoptotic process in UVB-induced sunburn keratinocytesProceedings of the National Academy of Sciences, 1999
- Galectins-1 and -3 and their ligands in tumor biologyZeitschrift für Krebsforschung und Klinische Onkologie, 1999
- Neoglycoproteins with the Synthetic Complex Biantennary Nonasaccharide or Its α2,3/α2,6-Sialylated Derivatives: Their Preparation, Assessment of Their Ligand Properties for Purified Lectins, for Tumor Cells in Vitro, and in Tissue Sections, and Their Biodistribution in Tumor-Bearing MiceBioconjugate Chemistry, 1997