CELL-SURFACE CHANGES IN MOUSE PERITONEAL-MACROPHAGES AFTER INDUCTION WITH PROTEOSE PEPTONE OR THIOGLYCOLLATE - (ROSETTES WITH SRBC-MEMBRANE RECEPTOR-RADIOSENSITIVITY-EFFECT OF COLCHICINE-GLUTARALDEHYDE FIXATION)

  • 1 January 1979
    • journal article
    • research article
    • Vol. 25  (3) , 156-168
Abstract
Some 5-6% of mouse PEC [peritoneal exudate cells] spontaneously formed rosettes with SRBC [sheep red blood cells]. This weak intercellular interaction is most likely mediated by a receptor for SRBC located on the surface of peritoneal macrophages. Manifestation of the receptor is influenced by the genetic background and the H-2 halotype of PEC donors; a high proportion of RFC [rosette-forming cells] was associated with the H-2s haplotype. PEC derived from intact mice markedly differed in morphological and functional characteristics from those of PP[proteose peptone]- or TG[thioglycollate]-pretreated donors. Formation of rosettes by induced macrophages depended on the time interval between stimulation and PEC harvesting and on the type of inducing agent; it was also radiosensitive and more responsive to the action of colchicine. TG-induced macrophages had a significantly reduced capacity to bind syngeneic lymphoid cells. The difference in adhesivity between intact and stimulated PEC could be abolished by glutaraldehyde prefixation. In vivo induction resulted in modified morphological and functional properties of macrophages, including transformation of their cell surface.