An Enzyme-Linked Immunosorbent Assay (Elisa) for The Primary Diagnosis of Foot-And-Mouth Disease Characterization and Comparison with Complement Fixation
Open Access
- 1 June 1984
- journal article
- research article
- Published by Springer Nature in Acta Veterinaria Scandinavica
- Vol. 25 (2) , 280-296
- https://doi.org/10.1186/bf03547271
Abstract
A sandwich type ELISA for foot-and-mouth disease (FMD) virus types O, A and C was established, using a combination of rabbit anti-146 S and guinea pig hyperimmune antibodies. This method was found to be highly efficient for the detection of both 146 S particles and 12 S subunits. The ELISA was approximately 500 times more sensitive than complement fixation (CF) when examining epithelial samples of FMD vesicles. An early primary diagnosis of FMD was obtained by both CF and ELISA in 19 out of 21 confirmed cases. The remaining 2 cases were initially negative in CF but positive in ELISA.Keywords
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