Determination of Carbamic Herbicides by High Performance Liquid Chromatography (HPLC). II. Chlorpropham

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Abstract
Two simple, precise and rapid reversed-phase high performance liquid chromatographic procedures, external and internal standard methods, are described for the determination of chlorpropham in emulsifiable concentrates. Samples are diluted with methanol and 4-nitro-diphenyl ether is added as the internal standard. Calibration and quantitation is made with the use of pure chlorpropham, with results obtained in absolute or relative amounts. Linearity in both cases is achieved from 0.1 to 15 .mu.g of chlorpropham, equivalent to 10 .mu.L injections of solutions of this chemical at concentrations of 1 .times. 10-2 to 1.5 g l-1. Methanol/water (60/40 vol/vol) at a flow of 2 mL min-1 is used as eluent and retention times are approximately 4.4 and 5.9 min, respectively, for chlorpropham and 4-nitro-diphenyl ether. Detection limit was 3.9 ng of chlorpropham, that is, 10 .mu.l of a solution at 3.9 .times. 10-4 g l-1. The internal standard method improves slightly the confidence limit and the relative standard deviation with regard to the external standard method.

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