Comparative, Quantitative Study of Lymphoid and Non-Lymphoid Uptake of 60 nm Polystyrene Particles
- 1 January 1994
- journal article
- research article
- Published by Taylor & Francis in Journal of Drug Targeting
- Vol. 2 (2) , 151-156
- https://doi.org/10.3109/10611869409015904
Abstract
Uptake by gut epithelial tissue of 60 nm polystyrene particles was studied in female Sprague-Dawley rats (180g, 9 weeks old) after 5 days oral dosing by gavage (14mg/kg). The gut was divided into lymphoid and non-lymphoid tissue of the small and large intestine, prior to analysis for polystyrene by gel permeation chromatography (GPC). Approximately 10% of the administered dose was recovered from the entire gastrointestinal tract. The total percentage of the administered dose taken up through lymphoid tissue was statistically much greater than through non-lymphoid tissue. It was estimated that 60% of the uptake in the small intestine occurred through the Peyer's patches, even though the patches comprised a small percentage of the total surface area of the small intestinal tissue. A significant amount of the total uptake was shown to occur in the large intestine, particularly in the lymphoid sections of this tissue. These results were confirmed by fluorescence microscopy.Keywords
This publication has 26 references indexed in Scilit:
- Further histological evidence of the gastrointestinal absorption of polystyrene nanospheres in the ratInternational Journal of Pharmaceutics, 1992
- Nanoparticle Uptake by the Rat Gastrointestinal Mucosa: Quantitation and Particle Size DependencyJournal of Pharmacy and Pharmacology, 1990
- Controlled vaccine release in the gut-associated lymphoid tissues. I. Orally administered biodegradable microspheres target the peyer's patchesJournal of Controlled Release, 1990
- The Uptake and Translocation of Latex Nanospheres and Microspheres after Oral Administration to RatsJournal of Pharmacy and Pharmacology, 1989
- The transport of microspheres from the gastro-intestinal tract to inflammatory air pouches in the ratJournal of Pharmacy and Pharmacology, 1989
- Intestinal absorption of RU 41740, an immunomodulating compound extracted from Klebsiella pneumoniae, across duodenal epithelium and Peyer's patches of the rabbitInternational Journal of Pharmaceutics, 1987
- Transmucosal passage of polyalkylcyanoacrylate nanocapsules as a new drug carrier in the small intestineBiology of the Cell, 1987
- Transit of pharmaceutical dosage forms through the small intestine.Gut, 1986
- The effect of oral immunization on the uptake of macromolecules by adult rat intestinal columnar epithelium and Peyer's patch tissue in vitroBiochemical Society Transactions, 1984
- Light and electron microscopy of intestinal ferritin absorption. Observations in sensitized and nonsensitized hamsters (Mesocricetus auratus)The Anatomical Record, 1966