HISTOCHEMISTRY OF THE DEVELOPING HUMAN BRAINI. Alkaline Phosphatase, Acid Phosphatase, and AS Esterase in the Cerebellum

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Abstract
In material of human fetuses as well as infants who had died during the neonatal period, with an even age distribution, the author studied alkaline and acid phosphatases as well as nonspecific esterases in the cerebellum. Comparison between fresh material and autopsy material revealed that the latter is applicable. It seems to be important that the bodies be refrigerated as soon as possible. Comparison of the Gomori and azo dye methods showed that[long dash]especially in autopsy material[long dash]these 2 methods give slightly different results. Alkaline phosphatases are demonstrated in endothelium and during the 1st 5 months in the luminal part of the ependymal cells where glycogen is also present. In the early fetal stages the acid phosphatases are localized to the ependymal cells and their processes, while later they appear in the nerve cells simultaneously with cytoplasmic basophilia. The differences in the distribution of acid phosphatases in Purkinje, basket, and Golgi cells are discussed. This enzyme may be observed also in glia cells and in perivascular cells. Esterases were found in approximately the same locations as the acid phosphatases, but more ample in the perivascular cells, less marked in the small nerve cells and not present in glia.

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