Antigen Binding to Cells: Determination by Enzymic Fluorogenic Group Hydrolysis
- 23 February 1968
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 159 (3817) , 884-885
- https://doi.org/10.1126/science.159.3817.884
Abstract
A sensitive method for detecting cells containing antibody to β-galactosidase has been devised. The enzyme attached to the cells containing antibody can hydrolyze a fluorogenic substrate and yield fluorescent products which are measured microphotofluorometrically. This method of detecting a few molecules of antibody is applicable to other enzyme antigen systems.Keywords
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