MORPHOLOGICAL, CHEMICAL, AND SEROLOGICAL STUDIES OF THE CELL WALLS OFRICKETTSIA MOOSERI

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Abstract
Cell walls of relative morphological purity were prepared from R. mooseri by controlled treatment with sodium desoxycholate. Chemical analyses revealed that these structures contain amino acids, polysaccharide and small amounts of ribonucleic acid. Twelve amino acids were identified in acid hydrolysates. No appreciable amounts of methionine, arginine and histidine were found. The polysaccharide moiety contains glucose, hexosamine, glucuronic acid and galactose. Complement fixation test demonstrated the presence of a group reactive antigen. An erythrocyte sensitizing substance was found in much greater amounts in the cell wall than in the rest of the organism.