STUDIES ON TOXIC LIPID OF ISONIAZID-RESISTANT HUMAN TUBERCLE BACILLI

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Abstract
Purified wax was extracted from isoniazid-resistant tubercle bacilli which were isolatedf rom a tuberculous patient. When this wax was fractionated into wax C and wax D, both fractions con-tained the toxic lipid, the higher concentration being in wax D. The toxic lipid was fractionated from purified wax by column chromatographv on silica gel-infusorial earth. The lipid was shown by means of chemical analysis and biologic activity to be identical with the cord factor of virulent tubercle bacilli. The toxic lipid content of isoniazid-resistant, attenuated tubercle bacilli was found to be of the same order as that of other virulent strains.