Electron Micrographs of Bacterial Cultures Infected with Bacteriophage

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Abstract
Collodion U.S.P. diluted with 5 or 6 volumes of amyl acetate is spread carefully over the growth surface of agar plates and drained till dry. When removed a replica of the plate is obtained, to which the colony layers and individual bacterial cells adhere. Contrast for electron microscopy may be increased by the vertical deposition of a few Angstroms thickness of aluminum, chromium or beryllium. The method is expected to aid in determining how bacteriophage acts on bacteria.

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