Isolation and characterization of a linear DNA plasmid from Streptomyces clavuligerus
- 1 April 1988
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 212 (1) , 172-176
- https://doi.org/10.1007/bf00322461
Abstract
A linear DNA plasmid (pSCL) has been isolated from Streptomyces clavuligerus by a method employing high concentrations of protease. Rate-zonal sedimentation on sucrose gradients was used to purify the plasmid. The plasmid is 12 kb in length and appears to be linked to protein at its 5′ termini. A restriction endonuclease map of the plasmid for ten enzymes has been determined. Evidence for terminally repeated sequences is provided by cross-hybridization analysis.Keywords
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