A Cyanobacterial Gene Encoding Peptidyl-Prolyl cis-trans Isomerase
Open Access
- 1 December 1992
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 100 (4) , 1982-1986
- https://doi.org/10.1104/pp.100.4.1982
Abstract
The rotA gene encoding peptidyl-prolyl cis-trans isomerase has been identified, sequenced, and shown to be transcribed in the cyanobacterium Synechococcus sp. PCC7942. Inactivation of the gene by replacement of a region containing the open reading frame with a gene conferring kanamycin resistance resulted in merodiploids containing both the wild type and the modified genomic region. We were not able to isolate a kanamycin-resistant mutant in which all the genomic wild-type copies were substituted, which suggests that such replacement could have been lethal.Keywords
This publication has 22 references indexed in Scilit:
- Protein folding in the cellNature, 1992
- Catalysis of protein folding by cyclophilins from different speciesJournal of Biological Chemistry, 1991
- Structure and expression of cytosolic cyclophilin/peptidyl-prolyl cis-trans isomerase of higher plants and production of active tomato cyclophilin in Escherichia coli.Proceedings of the National Academy of Sciences, 1990
- Cyclosporin A-binding protein (cyclophilin) of Neurospora crassa. One gene codes for both the cytosolic and mitochondrial forms.Journal of Biological Chemistry, 1988
- pl Bl5: A cDNA Clone of the Rat mRNA Encoding CyclophilinDNA, 1988
- Catalysis of protein folding by prolyl isomeraseNature, 1987
- High CO2 Requiring Mutant of Anacystis nidulans R2Plant Physiology, 1986
- Cyclophilin: distribution and variant properties in normal and neoplastic tissues.The Journal of Immunology, 1986
- Isolation and amino acid sequence of cyclophilin.Journal of Biological Chemistry, 1986
- The refolding of urea-denatured ribonuclease A is catalyzed by peptidyl-prolyl cis-trans isomeraseBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1985