A single-stranded DNA-binding protein promotes the binding of the purified oestrogen receptor to its responsive element
Open Access
- 1 January 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (19) , 5713-5716
- https://doi.org/10.1093/nar/18.19.5713
Abstract
The purified human oestrogen receptor (hER) does not form a detectable complex with an oestrogen responsive element (ERE) under conditions where hER-ERE complexes are readily formed with crude extracts from Hela or yeast cells expressing the hER. This Indicates that other factor(s) are necessary for ER-ERE binding. Such a ER DNA binding stimulatory factor (DBSF) has been purified from the yeast Saccharomyces cerevislae. It is a 45 kDa single-stranded DNA-binding protein (SSB) which cannot be substituted for by the purified E.coi SSB.This publication has 13 references indexed in Scilit:
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