Immunofluorescence stimulated emission depletion microscopy
- 19 October 2003
- journal article
- research article
- Published by Springer Nature in Nature Biotechnology
- Vol. 21 (11) , 1303-1304
- https://doi.org/10.1038/nbt897
Abstract
We report immunofluorescence imaging with a spatial resolution well beyond the diffraction limit. An axial resolution of approximately 50 nm, corresponding to 1/16 of the irradiation wavelength of 793 nm, is achieved by stimulated emission depletion through opposing lenses. We have demonstrated not only that an antibody-tagged label is stable enough to be recorded in this microscopy mode, but also that subdiffraction resolution can be obtained using a standard immunofluorescence preparation.Keywords
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