Nucleosomes occurring in protein‐free hybridoma cell culture Evidence for programmed cell death
- 24 June 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 284 (2) , 285-287
- https://doi.org/10.1016/0014-5793(91)80705-8
Abstract
In addition to monoclonal immunoglobulin. two kinds of nucleoproteins. NP1 and NP2, were isolated from the supernatants of hybridoma cultures set up in a protein‐free medium. As shown by SDS‐electrophoresis the two nucleoproteins shared a set or proteins (apparent M r 11000 to 15000). and differed in the DNA moiety (∼ 150 bp in NP1. ∼ 350 bp in NP2). The amino acid composition of the protein moiety confirmed the nucleosomal origin of NP1 and NP2. The findings support the view that in hybridoma cultures the cells undergo death by apoptosis, i.e. a programmed process characterized by initial fragmentation of chromatin.Keywords
This publication has 13 references indexed in Scilit:
- Electron microscopy of hybridoma cells with special regard to monoclonal antibody productionCytotechnology, 1990
- Cyclosporin A inhibits activation-induced cell death in T-cell hybridomas and thymocytesNature, 1989
- Antibodies to CD3/T-cell receptor complex induce death by apoptosis in immature T cells in thymic culturesNature, 1989
- Induction and activation of tissue transglutaminase during programmed cell deathFEBS Letters, 1987
- Iron compounds at high concentrations enable hybridoma growth in A protein-free mediumBiotechnology Letters, 1987
- Serum-free medium for hybridoma and parental myeloma cell cultivation: A novel composition of growth-supporting substancesImmunology Letters, 1984
- Structure and expression in L-cells of a cloned H4 histone gene of the mouseJournal of Molecular Biology, 1981
- The ultrasensitive silver “protein” stain also detects nanograms of nucleic acidsBiochemical and Biophysical Research Communications, 1981
- Cell Death: The Significance of ApoptosisPublished by Elsevier ,1980
- Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes.The Journal of Experimental Medicine, 1978