Binding of different antigen-enzyme and antibody-enzyme conjugates by intracellular antibodies in cytoplasm and Golgi complex of plasma cells
- 1 January 1985
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 83 (1) , 61-63
- https://doi.org/10.1007/bf00495301
Abstract
Intracellular immunoglobulins in plasma cells were characterized by antigen-enzyme conjugates and anti-immunoglobulin antibody-enzyme conjugates applied in a double immunocytochemical approach. After their assemblage, immunoglobulins in the cytoplasm of anti-TNP antibody producing plasma cells can be demonstrated both by TNP-enzyme conjugates and by anti-immunoglobulin (μ or γ chain specific) antibody-enzyme conjugates. Once arrived in the Golgi complex (GC) detection with TNP-enzyme conjugates remains possible, but anti-immunoglobulin antibody-enzyme conjugates did not bind to a detectable degree. Similar results were obtained in experiments where immunoglobulin-enzyme conjugates were used both as an antigen-enzyme conjugate and as an antibody-enzyme conjugate.Keywords
This publication has 14 references indexed in Scilit:
- Double immunocytochemical staining in the study of antibody-producing cells in vivo. Combined detection of antigen specificity (anti-TNP) and (sub)class of intracellular antibodies.Journal of Histochemistry & Cytochemistry, 1985
- Double immunocytochemical staining in the study of antibody-producing cells in vivo. Simultaneous detection of cells producing monospecific antibodies and cells producing cross-reacting antibodies in the spleen of rabbits after injection of two related antigens.Journal of Histochemistry & Cytochemistry, 1984
- Double immunocytochemical staining in the study of antibody-producing cells in vivo. Detection of specific antibody-producing cells in the spleen and simultaneous determination whether or not they produce immunoglobulin G antibodies.Journal of Histochemistry & Cytochemistry, 1984
- The development of specific antibody‐containing cells in the spleen of rabbits during the secondary immune response against free or liposome‐associated albumin antigenThe Anatomical Record, 1984
- Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes).Journal of Histochemistry & Cytochemistry, 1984
- Lectin-binding sites as markers of Golgi subcompartments: proximal-to-distal maturation of oligosaccharides.The Journal of cell biology, 1983
- Immunohistochemical characterization of intracellular J-chain and binding site for secretory component (SC) in human immunoglobulin (Ig)-producing cellsMolecular Immunology, 1983
- Plasma cell immunoglobulin M molecules. Their biosynthesis, assembly, and intracellular transport.The Journal of cell biology, 1979
- ULTRASTRUCTURAL LOCALIZATION OF ANTIBODY IN DIFFERENTIATING PLASMA CELLSThe Journal of Experimental Medicine, 1968
- CYTOCHEMICAL DEMONSTRATION OF PEROXIDASE ACTIVITY WITH 3-AMINO-9-ETHYLCARBAZOLEJournal of Histochemistry & Cytochemistry, 1965