Polymer supported DNA synthesis using hydroxybenzotriazole activated pbosphotriester intermediates
Open Access
- 1 January 1984
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 12 (22) , 8639-8651
- https://doi.org/10.1093/nar/12.22.8639
Abstract
Phosphorylation of N-acyl-5′-0-DMTr-d-nucleosides, or similarly protected DNA-dimers having a free 3′-0H group, with 2-chlorophenyl-0, 0-bis(1-benzotriazolyl)phosphate affords reactive 3′-phosphotriester derivatives. The latter intermediates can be used, without further purification, for the synthesis of DNA-fragments on the controlled pore glass/long chain alkylamine support. Further, 2-cyano-1, 1-dimethylethoxy dichlorophosphine proved to be very suitable for the preparation of 5′-phosphorylated DNA-fragments on the same type of solid support.Keywords
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