Restriction endonuclease mapping of the genome of feline herpesvirus type 1

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Abstract
Summary Restriction endonuclease mapping was used to determine the molecular structure of feline herpesvirus type 1 (FHV-1) strain B 927. FHV-1 DNA purified from infected cells by pulsed field gel electrophoresis was partially restricted with Sau 3A to generate a series of overlapping fragments of 10–20 kb. EMBL 4 DNA was digested with Bam HI and Sal I and viral DNA was inserted into the “stuffer” region. Recombinant phage were selected onE. coli strain Q 359. Single and double digestions with the enzymes Eco RI, Bam HI, Sal I, Sst I and Xho I enabled construction of physical maps of the genome. The FHV-1 genome is approximately 126 kbp in length and is composed of a long unique region of 99 kbp and a short region of 27 kbp comprising an unique sequence of 8–9 kbp flanked by inverted repeats of 7–8.5 kbp.