Thermal Inactivation Energy of Granulocyte-Monocyte Stem Cells

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Abstract
Hyperthermia inhibited the cellular proliferation of granulocyte-monocyte (G-M) stem cells from C3H mice in Millipore diffusion chamber culture. At temperatures of 42.5 and 43.5.degree. C the survival curves obtained by plotting heating time against inhibition of cellular proliferation showed an initial shoulder followed by an exponential decrease. Above 43.5.degree. C there was a rapid exponential decrease in cellular proliferation. From the Arrhenius plot of 1/D37 (D37 is the heating time required to reduce cellular proliferation by 63%) vs. 1/T (T is the absolute temperature) the thermal inactivation energy (u) of G-M precursors was 83 and 210 kcal/mole above and below 43.5.degree. C, respectively. These inactivation energies were lower than those for established cell lines; this may imply a difference in the subcellular target(s) of thermal injury. [Applicability of this study to the treatment of hematologic neoplasia is discussed.].