Analysis of large DMA from soybean (Glycine max L. Merr.) by pulsed‐field gel electrophoresis
Open Access
- 1 January 1992
- journal article
- research article
- Published by Wiley in The Plant Journal
- Vol. 2 (1) , 133-135
- https://doi.org/10.1046/j.1365-313x.1992.t01-53-00999.x
Abstract
The technique of pulsed‐field gel electrophoresis (PFE) has been used to study chromosomal regions and entire genomes of several organisms. Techniques are presented for the isolation of high molecular weight DMA from embedded soybean protoplasts and the conditions for separating large DMA fragments using PFE. Digestion was detected by Southern hybridization using single copy nodulin clones. These data are being used to generate a physical map of the nodulin region(s) of the soybean genome.Keywords
This publication has 19 references indexed in Scilit:
- Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations.Proceedings of the National Academy of Sciences, 1991
- ED: pulsed electrophoresis instrumentNature, 1989
- Pulsed field gel electrophoresis and physical mapping of large DNA fragments in the Tm-2a region of chromosome 9 in tomatoMolecular Genetics and Genomics, 1989
- Preparation of plant DNA for separation by pulsed field gel electrophoresisElectrophoresis, 1989
- A Physical Map of the Escherichia coli K12 GenomeScience, 1987
- Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresisCell, 1984
- Nuclear DNA amounts in angiospermsProceedings of the Royal Society of London. B. Biological Sciences, 1982
- Nuclear DNA amounts in angiospermsPhilosophical Transactions of the Royal Society of London. B, Biological Sciences, 1976
- The Use of Fluorescein Diacetate and Phenosafranine for Determining Viability of Cultured Plant CellsStain Technology, 1972
- Nutrient requirements of suspension cultures of soybean root cellsExperimental Cell Research, 1968