Characterization of Mixed Enzyme-Mediator-Carbon Paste Electrodes
- 1 July 1993
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 26 (7) , 1281-1299
- https://doi.org/10.1080/00032719308017413
Abstract
Amperometric bioelectrodes based on carbon paste mixed with glucose oxidase (GOX) and dimethylferrocene (DMFc), are characterized by using cyclic voltammetry, potential step chronoamperometry and amperometry at a rotating disk electrode. The catalytic process appears to be highly dependent on the amount of DMFc. Kinetic control, internal diffusion control and external diffusion control can be obtained only by varying the amount of incorporated DMFc. It was shown that the Eadie-Hofstee plot is a particularly useful tool in diagnosis of diffusional limitations. The high efficiency of electrochemical regeneration of high amounts of DMFc∗ surrounding GOX solves the problem of co-substrate transport. The catalytic oxidation current of ascorbic acid was highly diminished by casting a lipid/Nafion membrane and totally eliminated by treatment of the sample with hydrogen peroxide prior to measurement.Keywords
This publication has 36 references indexed in Scilit:
- Preparation and characterization of a fragile enzyme immobilized carbon paste electrodeBioelectrochemistry and Bioenergetics, 1992
- Carbon-Paste Electrodes with Incorporated Lactate Oxidase and MediatorsAnalytical Letters, 1992
- Long-Term Operational Stability of a Mixed Glucose Oxidase-Redox Mediator-Carbon Paste ElectrodeAnalytical Letters, 1991
- A glucose electrode based on carbon paste chemically modified with a ferrocene-containing siloxane polymer and glucose oxidase, coated with a poly(ester-sulfonic acid) cation-exchangerAnalytica Chimica Acta, 1990
- Electrochemical Behaviour of a Lipid Modified Enzyme ElectrodeAnalytical Letters, 1989
- Amperometric Enzyme Based Biosensor For the Detection of Xanthine Via SuperoxideAnalytical Letters, 1989
- Decreased ascorbate sensitivity with nafion-coated carbon fibre electrodes in combination with copper(II) ions for the electrochemical determination of electroactive substances in vivoAnalytica Chimica Acta, 1987
- Mediated amperometric biosensors for d-galactose, glycolate and l-amino acids based on a ferrocene-modified carbon paste electrodeAnalytica Chimica Acta, 1986
- Electrocatalytic oxidation of glucose at a glucose oxidase-immobilized benzoquinone-mixed carbon paste electrode.Agricultural and Biological Chemistry, 1986
- Effect of internal diffusion in heterogeneous enzyme systems: Evaluation of true kinetic parameters and substrate diffusivityJournal of Theoretical Biology, 1973