Tris: an allosteric effector of tarantula haemocyanin
- 14 February 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 339 (1-2) , 37-39
- https://doi.org/10.1016/0014-5793(94)80379-x
Abstract
The effect of the chemical buffering component Tris (hydroxy-methyl-amino-methane) and of chloride ions on the oxygen binding of tarantula hemocyanin was studied at constant pH. It revealed that Tris at micromolar concentrations decreases the oxygen pressure at half-saturation (P50) by a factor of more than two, whereas chloride does not influence oxygen affinity. A thermodynamic analysis in terms of the nested model of allostery [(1987) Proc. Natl. Acad. Sci. 84, 1891-1895] indicated that Tris acts a an allosteric activator of oxygen binding by influencing the interaction between the 12-meric half-molecules of the 24-meric tarantula haemocyaninKeywords
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