Plasma Membrane Ultrastructural Differences Between the Exponential and Stationary Phases of Saccharomyces cerevisiae as Revealed by Freeze-Etching

Publisher Website
Google Scholar
Abstract
Ultrastructural changes in the plasma membrane of S. cerevisiae during the exponential and stationary growth phases were studied by freeze-etching. In the exponential phase plasma membrane-intercalated particles were distributed randomly. In the stationary phase several areas of the plasma membrane showed a hexagonal arrangement of particles; these areas appeared to increase with the age of the culture. The polarity of the particles changed partially: the E-face [concave fracture face] of the plasma membrane was sparsely embedded with particles in exponential phase cells, but relatively densely embedded in stationary phase cells. Invaginations of the plasma membrane on the P-face [convex fracture face] were devoid of particles during both growth phases. Invaginations of the E-face were sparsely embedded with particles in exponential phase cells, but densely embedded with particles in stationary phase cells.

This publication has 4 references indexed in Scilit: