UTILIZATION OF ACETATE, PYRUVATE, AND CO2BY PENICILLIUM DIGITATUM

Abstract
The utilization of C14specifically labelled pyruvate, acetate, and CO2by proliferating Penicillium digitatum cells has been studied by means of a series of time-course experiments. Pyruvate appeared to be utilized by way of acetate and the latter was in turn utilized mainly via the TCA processes. Degradation studies on aspartic acid and glutamic acid isolated from cells grown on acetate-1-C14or C14O2indicated that CO2can be fixed in this organism by means of a two-stage process onto a "C2unit"; the latter is thought to be derived from glyoxalic acid produced in the "glyoxalate bypass" pathway.