Abstract
Total ultraviolet-absorbing constituents of wort and beer are partially resolved by direct gel column analysis, with continuous monitoring of effluent at 280 nm. The elution profile enables routine assessment to be made of the protein-phenolic fraction, of nucleic acid derivatives and of tryptophan, together with a measure of the generally low level of monomeric phenolics. The major portion of total ultraviolet absorbance in these brewing materials is apparently due to nucleic acid derivatives, which originate from the mashing process.

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