Production of H-Y antibody in the ascites fluid of mouse and localization of the antigen on cells and tissues
- 1 January 1989
- journal article
- research article
- Published by Wiley in Gamete Research
- Vol. 22 (1) , 37-49
- https://doi.org/10.1002/mrd.1120220105
Abstract
A procedure is described for the production of large amounts of ascites fluid containing specific H-Y antibody. The distribution of H-Y antigen on mouse epididymal spermatozoa, thymocytes, and splenocytes was carried out using this specific antibody in the microcytotoxicity test and ELISA. Employing the indirect immunofluorescent technique, the H-Y antigen was localized on the acrosomal membrane of mouse epididymal and washed ejaculated human spermatozoa and on the entire membrane of mouse splenocytes and thymocytes. Immunohistochemical localization of the antigen in the testicular section indicated its presence in the cytoplasm of Leydig cells and on the membrane of Sertoli cells and sperm heads.Keywords
This publication has 18 references indexed in Scilit:
- Separation of the genetic loci for the H–Y antigen and for testis determination on human Y chromosomeNature, 1987
- Spermatogenic failure in male mice lacking H–Y antigenNature, 1986
- H-Y antigen and sex determinationImmunology Today, 1985
- Serologically detectable H-Y (‘male’) antigen: Mr or myth?Immunology Today, 1985
- The Y-Linked Testis Determining Gene and H-Y Plasma Membrane Antigen Gene: Are They One and the Same?*Endocrine Reviews, 1985
- Sexual differentiation and H-Y antigenNature, 1982
- Serology of H-Y antigenHuman Genetics, 1981
- DETECTION OF H-Y ANTIGEN BY FLUORESCENCE MICROSCOPY1Transplantation, 1978
- PEROXIDASE-LABELED ANTIBODY A NEW METHOD OF CONJUGATIONJournal of Histochemistry & Cytochemistry, 1974
- Topographical Location of H—Y Antigen on Mouse Spermatozoa by ImmunoelectronmicroscopyProceedings of the National Academy of Sciences, 1973