Pathogen detection using short-RNA deep sequencing subtraction and assembly
Open Access
- 11 June 2011
- journal article
- research article
- Published by Oxford University Press (OUP) in Bioinformatics
- Vol. 27 (15) , 2027-2030
- https://doi.org/10.1093/bioinformatics/btr349
Abstract
Motivation: Early and accurate detection of human pathogen infection is critical for treatment and therapeutics. Here we describe pathogen identification using short RNA subtraction and assembly (SRSA), a detection method that overcomes the requirement of prior knowledge and culturing of pathogens, by using degraded small RNA and deep sequencing technology. We prove our approach's efficiency through identification of a combined viral and bacterial infection in human cells. Contact:nshomron@post.tau.ac.ilKeywords
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