Concentration of Hematopoietic Progenitor Cells from Human Bone Marrow by a New Type of Blood Component Separator

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Abstract
A new type of blood component separator (BCS) was used for the isolation of hematopoietic progenitor cells from human bone marrow aspirates. The BCS was filled with 100–150 ml bone marrow and centrifuged to prepare a buffy coat. This buffy coat was isolated in 10–15% of the original bone marrow volume and contained 64 ± 8% of the nucleated cells (NC). Morphological examination revealed that the buffy coat was highly enriched for myeloblasts, promyelocytes, lymphocytes and monocytes, whereas the contamination with granulocytes was reduced to 46 ± 8% of the granulocytes initially present in the bone marrow suspension. In addition the contamination with red blood cells (RBC) was very low; the buffy coat contained only 6 ± 2% of the RBC. Furthermore it was demonstrated by means of colony assays that the buffy coat was highly enriched for hematopoietic progenitor cells. It contained 91 ± 6% of the granulocyte/monocyte progenitor cells (CFU‐GM) and 87 ± 9% of the erythroid progenitor cells (BFU‐E). These results are comparable to those obtained with continuous or semicontinuous blood cell processors. The advantages of the BCS is that it is a simple and inexpensive apparatus which fits in a normal blood bank centrifuge. It permits efficient preparation and isolation of a buffy coat from human bone marrow without substantial loss of hematopoietic progenitor cells.

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