Integrated stereological and biochemical studies on hepatocytic membranes. I.V. Heterogeneous distribution of marker enzymes on endoplasmic reticulum membranes in fractions.

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Abstract
The relationships between the surface area of the endoplasmic reticulum (ER) and constituent marker enzyme activities were considered quantitatively, particularly their occurrence in fractions collected from rat liver homogenates. The ER surface area was estimated in 5 membrane-containing fractions by use of a combined cytochemical-stereological technique, while ER marker enzymes were assayed biochemically. Fraction/homogenate recoveries for the ER enzymes averaged 100%, total membrane surface area 98% and ER surface area 96%. Relative specific activities, which compare the relative amounts of ER marker enzyme activities to the relative ER surface area in the membrane-containing fractions, indicate variable distributions for glucose-6-phosphatase and NADPH cytochrome c reductase, but not for esterase.

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