Adeno-Associated Virus Studies Employing A Fluorescent Focus Assay Technique

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Abstract
AAV antigens could not be recognized in KB cells by immuno-fluorescence unless the cells had been infected with adenoviruses. Of the adenoviruses studied, (types 1, 2, 4, 6 and 12) type 12 was the most efficient helper virus. A measurable fraction of adenovirus infected cells failed to produce recognizable AAV antigen(s). AAV super-infection suppressed the number of adenovirus infected cells producing recognizable adenovirus antigens. The fluorescent focus assay method employed was at least 1,000 times more sensitive than electron microscopy and 10,000 times more sensitive than complement fixation for detecting AAV.