Immunogenicity of a hybridPlasmodium falciparum, malaria antigen
- 6 April 1993
- journal article
- research article
- Published by Cambridge University Press (CUP) in Parasitology
- Vol. 106 (5) , 451-457
- https://doi.org/10.1017/s0031182000076733
Abstract
SUMMARY: A recombinant baculovirus-expressed hybrid protein containing epitopes for the C-terminal fragment of thePlasmodium falciparumprecursor to the major merozoite surface antigens (PMMSA) and the tetrapeptide repeats of the circumsporozoite protein (CSP) was assessed for its immunogenicity. Murine MHC-II restriction of the antibody response to the CSP repeats was not overcome by the PMMSA component, the response to which showed no restriction. In an adjuvant trial the highest antibody titres in rabbits to both components of the hybrid were obtained using Freund's adjuvant. Lack of a boosting antibody response to the CSP repeats appeared to be linked to the conformation of the PMMSA component. Formulation of the hybrid protein into Iscoms gave antibody titres of only short duration to both components.Keywords
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