Evidence thatpheV, a gene for tRNAPheofE. coliis transcribed from tandem promoters
Open Access
- 1 January 1985
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 13 (10) , 3699-3710
- https://doi.org/10.1093/nar/13.10.3699
Abstract
A DNA fragment of 487 bp containing a gene for tRNAPhe has been sequenced. Although the tRNAPhe sequence is identical to that of pheU (which maps at 94.5 min) the surrounding sequences are quite different. This sequence is thus that of a second gene for tRNAPhe (which we shall call pheV) In vitro transcription experiments and S1 mapping in vivo show the existence of two promoters separated by about 60 nucleotides. The second transcript starts only 3 nucleotldes 5′ from the tRNAPhe structural sequence. A DNA sequence characteristic of a rho-independent terminator is located 30 nucleotides 3′ of the end of the structural gene and is shown to function efficiently in vitro.Keywords
This publication has 18 references indexed in Scilit:
- [57] Sequencing end-labeled DNA with base-specific chemical cleavagesPublished by Elsevier ,2004
- Identification of transfer RNA suppressors in Escherichia coliJournal of Molecular Biology, 1984
- REGULATION OF THE SYNTHESIS OF RIBOSOMES AND RIBOSOMAL COMPONENTSAnnual Review of Biochemistry, 1984
- tRNAMetf2 gene in the leader region of the nusA operon in Escherichia coli.Proceedings of the National Academy of Sciences, 1984
- Conserved features of coordinately regulatedE.colipromotersNucleic Acids Research, 1984
- Regions of DNA involved in the stringent control of plasmid-encoded rRNA in vivoCell, 1983
- Differential stringent control of the tandem E. coli ribosomal RNA promoters from the rrnA operon expressed in vivo in multicopy plasmidsCell, 1983
- Organization and structure of an E. coli tRNA operon containing seven tRNA genesCell, 1981
- Attenuation and processing of RNA from the rplJL--rpoBC transcription unit of Escherichia coli.Proceedings of the National Academy of Sciences, 1980
- REGULATORY SEQUENCES INVOLVED IN THE PROMOTION AND TERMINATION OF RNA TRANSCRIPTIONAnnual Review of Genetics, 1979