BOVINE CON A-INDUCED SUPPRESSOR CELLS - GENERATION, MACROPHAGE REQUIREMENTS AND POSSIBLE MECHANISMS OF REGULATORY ACTION

  • 1 January 1981
    • journal article
    • research article
    • Vol. 43  (1) , 91-100
Abstract
Bovine concanavalin [Con] A-induced suppressor cells were generated from lymphocytes which were non-adherent to anti-Ig coated dishes and cells possessing receptors for peanut agglutinin. Bovine lymphocytes, preincubated with 25 .mu.g/ml of Con A for 40-45 h, could suppress the responses of autologous cells to the mitogens Con A, phytohemagglutinin and pokeweed mitogen as much as 90% when they were cultured together at a ratio of 1:1 (suppressor cell to responder cell) or higher. Suppressor cells were not necessary at the initiation of the mitogenic assay as they could regulate responding cells if added at 48 h in a 72 h assay. Allogeneic responder cells could be suppressed at the same level as autologous cells indicating a lack of genetic restriction. Macrophages were not required for suppressor cell generation because peripheral blood lymphocytes (PBL) depleted of macrophages by Sepharose G-10 columns, and subsequently incubated with Con A, could suppress autologous cells to a similar degree as unseparated PBL. Responder cells depleted of macrophages had normal mitogen responsiveness and were suppressed indicating macrophages were not required in transmission of suppressor signals. Cell-to-cell contact was not required for suppression connoting a soluble factor(s) as the suppression modulator.

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