DNA bending and binding factors of the human β-actin promoter
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (2) , 523-537
- https://doi.org/10.1093/nar/17.2.523
Abstract
Transcription of the .beta.-actin gene is rapidly inducible in response to serum stimulation. To determine the regions responsible for serum inducible and basal level expression, the human .beta.-actin promoter was subjected to mutational analysis. Two distinct elements, the CCAAT homology and the .beta.-actin specific conserved sequences, were found by a chloramphenicol acetyltransferase expression assay and sequence comparisons, and then analyzed for possible functions. Using a DNA bend assay, it was shown that the conserved sequences included the core of a sequence-directed bend of DNA. Gel mobility shift and DNase I protection assays revealed that the conserved sequences and the CCAAT homology were recognized by binding factors in HeLa cell extracts.Keywords
This publication has 41 references indexed in Scilit:
- DNA sequence determinants of CAP-induced bending and protein binding affinityNature, 1988
- Purified transcription factor AP-1 interacts with TPA-inducible enhancer elementsCell, 1987
- The integration host factor of Escherichia coli binds to bent DNA at the origin of replication of the plasmid pSC101Cell, 1987
- Transcriptional selectivity of viral genes in mammalian cellsCell, 1986
- An altered DNA conformation in origin region I is a determinant for the binding of SV40 large T antigenCell, 1986
- Two distinct transcription factors bind to the HSV thymidine kinase promoter in vitroCell, 1985
- Induction of c-fos gene and protein by growth factors precedes activation of c-mycNature, 1984
- Platelet-derived growth factor induces rapid but transient expression of the c-fos gene and proteinNature, 1984
- Conformational change in the DNA associated with an unusual promoter mutation in a tRNA operon of SalmonellaCell, 1984
- Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogeneNature, 1984