Separation of cryptosporidium oocysts from fecal debris by density gradient centrifugation and glass bead columns

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Abstract
A method was developed to obtain purified Cryptosporidium oocysts from fecal samples. Oocysts were initially collected by centrifugation through a sucrose density gradient and further purified by passage through glass bead columns. The purified oocysts were antigenically active and sufficiently pure for immunological studies.