Bacillussubtilis holo‐cytochrome c‐550 can be synthesised in aerobic Escherichia coli
- 17 September 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 270 (1-2) , 147-151
- https://doi.org/10.1016/0014-5793(90)81255-m
Abstract
Bacillus subtilis membrane-bound holo-cytochrome c-550 was found to be expressed from the structural gene cloned on a plasmid vector in aerobically grown Escherichia coli and exhibited normal biochemical properties. This occurs despite the lack of endogenous eytochrome c and suggests that eytochrome c-heme lyase activity is also present in aerobic E. coli. The membrane topology of B. subtilis eytochrome c-550 was studied using fusions to alkaline phosphatase (PhoA). The results show that the heme domain (at least when fused to PhoA) can be translocated as apo-cytochrome and confirm that the N-terminal part of the cytochrome functions as both export signal and membrane anchor for the C-tenninal heme domain. A model for the organisation of B. subtilis cytochrome c-550 in the cytoplasmic membrane is presented.Keywords
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