CYTOTOXICITY OF AN I-125-LABELED DNA-BINDING COMPOUND THAT INDUCES DOUBLE-STRANDED DNA BREAKS
- 1 January 1979
- journal article
- research article
- Vol. 39 (8) , 3244-3247
Abstract
[125I]Iodorivanol (6,9-diamino-2-ethoxy-5-[125I]iodoacridine) was prepared by direct iodination of rivanol (6,9-diamino-2-ethoxyacridine). In vitro binding of [125I]iodorivanol to phage PM2 DNA resulted in induction of double-stranded DNA breaks following decay of 125I atom, presumably in the same way as decay of 125I atoms in 125I-labeled DNA causes double-stranded DNA breaks. Treatment of mouse L-cell cultures with [125I]iodorivanol resulted in a cell kill, the extent of which was dependent on the 125I specific activity and the duration of exposure. A clonogenic assay was used to quantitate cell kill. At least some of the [125I]iodorivanol in the culture medium was taken up by the cells, transported to the nucleus and bound to DNA, and subsequent decay of the 125I atoms induced double-stranded DNA breaks in the genome with consequent loss of viability. 125I-labeled DNA-binding compounds are suggested as a novel class of cytotoxic agents.This publication has 5 references indexed in Scilit:
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