The Role of pH of the Mobile-Phase in Ion-Interaction RP-HPLC
- 1 December 1994
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 17 (20) , 4365-4380
- https://doi.org/10.1080/10826079408013623
Abstract
The dependence of retention on pH of the mobile phase is investigated in reversed-phase ion interaction chromatography, being the mobile phase an aqueous solution of 5.0 mM octylammonium and ortho-phosphoric acid at different pH values. The analytes considered were amines, diamines, amides, and species characterized by different functionalities. The behaviour of retention as a function of pH is discussed with comparison with literature data and correlation with pka values is shown. The optimization of separation and resolution in a mixture of acids and amines through pH variations is also presented.Keywords
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