Indirect Staining Reaction with Fluorescent Antibody for Detection of Antibodies to Pathogenic Fungi

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Abstract
Antibodies in sera from patients with systemic mycoses were demonstrated by the indirect method of staining with fluorescent antibody. In spite of a general autofluorescence of pathogenic fungi, addition of human anti-serum and a fluorescein-labeled rabbit antihuman gamma globulin resulted in characteristic fluorescing reactions. Cryptococcosis antibodies were demonstrated by the fluorescent technique but not by other serologic methods.