Chemical characteristics of D-I, a hypotensive factor occurred in acetone extract of bovine brain.

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Abstract
A modified procedure for preparation of D-I is presented and yields highly purified material showing a single spot on TLC more effectively. Examinations on chemical characteristics of D-I involving assessments of functional groups by chemical inactivations treated with various agents were performed. In Girard''s separation, about 30% of depressor-activity was recovered in nonketonic fraction. By treatment with sodium borohydride, the hypotensive activity disappeared completely. It was abolished completely with treatment by hydrogen peroxide (0.1%) or permanganate, while the activity was recovered almost completely by treatment with ferric chloride. It was also damaged with bromine. D-I became substantially less active by treatment with succinic anhydride, and inactive by phenylisocyanate. D-I was unaffected by treatment with triphenylphosphine or diazomethane at cold. The attitudes of D-I against several spray reagents on TLC were examined and 2,4-dinitrophenylhydrazine-ferricyanide, 2'',7''-dichlorofluorescein and others were found useful for D-I detection. Absorptional pattern of D-I in infrared spectrum resembled that of lysophosphatidyl choline D-I may be a choline-containing phospholipid having double bond, free hydroxyl or ester. It has no free amino, free carboxyl, amide, peroxide or vicinal hydroxyl group and it may have no carbonyl group other than ester. It may be readily convertible to a ketonic compound.