Insertion of DNA sequences into the human chromosomal β-globin locus by homologous recombination
- 1 September 1985
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 317 (6034) , 230-234
- https://doi.org/10.1038/317230a0
Abstract
A ''rescuable'' plasmid containing globin gene sequences allowing recombination with homologous chromosomal sequences has enabled us to produce, score and clone mammalian cells with the plasmid integrated into the human .beta.-globin locus. The planned modification was achieved in about one per thousand transformed cells whether or not the target gene was expressed.Keywords
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