Feline sarcoma virus-coded polyprotein: enzymatic cleavage by a type C virus-coded structural protein

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Abstract
A purified 15,000-MW Prague strain Rous sarcoma virus gag gene-coded structural protein, p15, was shown to enzymatically cleave the previously described 130,000 MW feline sarcoma virus-coded polyprotein, Pr130. Cleavage products included proteins ranging in MW from 12,000-110,000. The specificity of this cleavage reactivity was indicated by the fact that, under similar conditions, neither purified type C viral structural proteins nor nonviral proteins such as bovine serum albumin were cleaved to significant extents. Moreover, feline leukemia virus Pr65gag was efficiently cleaved, resulting in the generation of proteins of 30,000 (p30), 15,000 (p15), 12,000 (p12) and 10,000 (p10). Feline sarcoma virus Pr[protein]130 was used enzymatically as starting material, a major 72,000 MW cleavage product was purified and it contained the previously described feline sarcoma virus-coded nonstructural component.