Blood-free selective medium for isolation of Campylobacter jejuni from feces

Abstract
A blood-free selective agar is described which contains charcoal, ferrous sulfate, sodium pyruvate, casein hydrolysates, cefazolin and sodium deoxycholate (CCD agar). CCD agar was compared with Preston medium for isolation of C. jejuni from human feces, and isolation rates were similar on both media, but CCD agar was less selective. Temperature studies at 37.degree. and 42.degree. C confirmed that incubation of direct plates at 42.degree. C for 48 h was necessary for maximum isolation of C. jejuni.