Electron microscopic study of the cell surface of dibekacin-treated Pseudomonas aeruginosa.
- 1 January 1986
- journal article
- research article
- Published by Tohoku University Medical Press in The Tohoku Journal of Experimental Medicine
- Vol. 150 (1) , 69-75
- https://doi.org/10.1620/tjem.150.69
Abstract
Electron microscopy of thin sections of Pseudomonas aeruginosa IAM 1007 treated with dibekacin revealed blebs, disintegration of outer membrane of the cell wall and degenerative features of the cytoplasm. In the next experiment, the cell wall fraction was isolated from the mechanically disrupted cells, incubated with dibekacin and was subjected to electron microscopy, in order to find a clue to the action mechanism of dibekacin on the cell wall of Pseuduomonas aeruginosa IAM 1007. As a result, it was found that unidentified substances were released from the surface of the cell wall and that the outer membrane of the cell wall disappeared. The degree of changes of the cell wall ultrastructure was almost proportional to the length of incubation with dibekacin. These findings strongly suggest that dibekacin directly disintegrates the outer membrane of the cell wall of Pseudomonas aeruginosa.This publication has 1 reference indexed in Scilit:
- Intracellular Distribution of 3 H-Dihydrostreptomycin in a Streptomycin-dependent Strain of Bacillus megateriumJournal of Bacteriology, 1968