Intracellular Distribution of 3 H-Dihydrostreptomycin in a Streptomycin-dependent Strain of Bacillus megaterium

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Abstract
The cells of a streptomycin-dependent strain of B. megaterium took up only 2-5% of the dihydrostreptomycin present in the medium when grown in the minimum concentration of streptomycin required for growth. During growth in the presence of 3H-dihydrostreptomycin, radioactivity was accumulated intracellularly in 3 forms, unbound, loosely bound to the ribosomes (removable by dialysis) and tightly bound to the ribosomes (retained after prolonged dialysis). More radioactivity for a given amount of RNA was bound by ribosomes attached to the cell membrane than by supernatant ribosomes. Of the nondialyzable radioactivity associated with isolated RNA, 40-60% was solubilized by treatment with ribonuclease or by dilute alkaline hydroylsis.